Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
GenDEPOT TBST(10X), pH 7.4 | 1L
TBST(10X) is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays. The 10X TBST (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.14M NaCl, 0.1% Tween-20, pH 7.4, when diluted to 1X with water.
Hampton Research HR2-727/1.0 M TRIS hydrochloride pH 8.5 - 100 ml
HR2-727/1.0 M TRIS hydrochloride pH 8.5 - 100 ml. Please note items from this supplier are non-returnable.
Gold Biotechnology Inc Tris (Tris Base) 500 g
GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Biotium TBE Buffer, 5X
5X TBE (Tris-Borate-EDTA) is a concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis. 1X solution: 89 mM Tris,89 mM boric acid, 2 mM EDTA. Store at room temperature.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.6 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.6 (0.2 micron filtered), 500 ml.
Nusep Inc NUVIEW MINI GEL 4-20 12-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 4-20% 12well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Bioworld Tris/Glycine Buffer for Resolve Gels (1X), 40 PK(s)
1X Tris/Glycine Buffer for Resolve GelsBuffer System: Pre-weighed powder mix in pouches (packs)1 pouch(pack)= 500 mL
Zymo Research Corporation DNA Elution Buffer, 50ml
This can be used with any of the DNA purification for DNA elution from columns, plates, and magbeads.
Sigma Aldrich Fine Chemicals Biosciences Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | 77-86-1 | MFCD00004679 | 100G
Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | Purity: >=99.8%; 99.8-100.1% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Teknova 10X TBS, pH 7.4. 1000mL, Sterile (247mM Tris, 27mM KCl, 1.37M NaCl) 2 pack.
247mM Tris-HCl,27mM Potassium Chloride,1.37M Sodium Chloride
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid >=98.0% (KT) | 60-00-4 | MFCD00003541 | 50G
Ethylenediaminetetraacetic acid >=98.0% (KT) | Purity: >=98.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 50G
Teknova 10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.